强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

岛屿海岸线自动化提取及其时空变迁研究——以大连市獐子岛为例   总被引：1，自引：1，他引：0  

丁小松  单秀娟  陈云龙  金显仕  杨涛 《海洋科学》2018,42(2):46-55

海岸带是受人类活动和全球海平面上升影响的敏感地带,海岸线的提取和监测是海岸带生态系统研究和社会管理的重要内容。本文在遥感和地理信息系统的支持下,以修正的归一化水体指数(Modified Normalized Difference Water Index,MNDWI)为基础,结合遥感影像处理和直方图均衡化等技术,实现了大连市獐子岛1985—2016年海岸线的自动化提取。结果表明:(1)通过与三位专家目视解译的成果比对,本文提取海岸线的精度能满足后续研究的要求(相对误差分别为0.045%,0.032%和0.023%);(2)近30年来,獐子岛海岸线总体呈现蚀退趋势,岸线长度与岛屿面积分别呈现变短和变小的趋势,獐子岛(主岛)和大耗岛的岸线蚀退速率最大,褡裢岛次之,小耗岛最小;在人类活动较为密集的区域,海岸线呈现出较为强烈的增长趋势,海水养殖和圈海建坝是岸线增长的主要驱动力;(3)獐子岛海岸线具有显著的分形性质,分形维数随时间呈现增大的趋势,獐子岛(主岛)的分形维数最大,褡裢岛的分形维数最小。  相似文献   

A critical state model for sands dependent on stress and density     

Y.P. Yao  D.A. Sun  T. Luo 《国际地质力学数值与分析法杂志》2004,28(4):323-337

An elastoplastic model for sands is presented in this paper, which can describe stress–strain behaviour dependent on mean effective stress level and void ratio. The main features of the proposed model are: (a) a new state parameter, which is dependent on the initial void ratio and initial mean stress, is proposed and applied to the yield function in order to predict the plastic deformation for very loose sands; and (b) another new state parameter, which is used to determine the peak strength and describe the critical state behaviour of sands during shearing, is proposed in order to predict simply negative/positive dilatancy and the hardening/softening behaviour of medium or dense sands. In addition, the proposed model can also predict the stress–strain behaviour of sands under three-dimensional stress conditions by using a transformed stress tensor instead of ordinary stress tensor. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

On the practice of estimating fractal dimension   总被引：11，自引：0，他引：11  

James R. Carr  William B. Benzer 《Mathematical Geology》1991,23(7):945-958

Coastlines epitomize deterministic fractals and fractal (Hausdorff-Besicovitch) dimensions; a divider [compass] method can be used to calculate fractal dimensions for these features. Noise models are used to develop another notion of fractals, a stochastic one. Spectral and variogram methods are used to estimate fractal dimensions for stochastic fractals. When estimating fractal dimension, the objective of the analysis must be consistent with the method chosen for fractal dimension calculation. Spectal and variogram methods yield fractal dimensions which indicate the similarity of the feature under study to noise (e.g., Brownian noise). A divider measurement method yields a fractal dimension which is a measure of complexity of shape.  相似文献   

Influence of Structural Non-Stationarity of Surface Roughness on Morphological Characterization and Mechanical Deformation of Rock Joints   总被引：2，自引：2，他引：0  

N. Fardin 《Rock Mechanics and Rock Engineering》2008,41(2):267-297

Summary Structural non-stationarity of surface roughness affects accurate morphological characterization as well as mechanical behaviour of rock joints at the laboratory scale using samples with a size below the stationarity threshold. In this paper, the effect of structural non-stationarity of surface roughness is investigated by studying the scale dependence of surface roughness and mechanical behaviour of rock joints. The results show that the structural non-stationarity mainly affects the accurate characterization of the surface roughness of the fracture samples. It also controls the amount and location of the contact areas during shear tests, which in turn affects the mechanical properties and asperity degradation of the samples. It is concluded that for accurate determination of the morphological and mechanical properties of rock joints at laboratory and field scales, samples with size equal to or larger than the stationarity threshold are required. Author’s address: Nader Fardin, Rock Mechanics Group, Department of Mining Engineering, Faculty of Engineering, University of Tehran, P.O. Box: 11365/4563, Tehran, Iran  相似文献   

Fractal Determination of Coastal Types Based on GIS--A Case Study of China     

Zhu xiaohua  WU Xiuqin  Wang Jian 《海洋通报(英文版)》2002,4(2)

The probability of fractal determination of coastal types based on GIS is preliminarily discussed with China as an example. Finally, some significant conclusions are drawn: (1) The fractal dimension of coastline of the bedrock coast is larger than that of the plain coast on the same scale map; (2) As far as the bedrock coast is concerned, the larger fractal dimension of coastline of the bedrock coast on the same scale map indicates that the bedrock coast is probably not typical; (3) As far as the plain coast is concerned, the smaller fractal dimension of coastline of the plain coast on the same scale map indicates that it is probably the silt plain coast; (4) The different substantial compositions affect the fractal dimensions of coastlines of different coastal types. In general, the coast which lies in the north of the Hangzhou Bay consists of sand mainly, its surface is flat, and it is connected with the coastal plain, its landform is broad shoal, its total change is comparatively homogenous in the tidal dynamic process, and thus, the relatively smaller fractal dimension of coastline results from this. For the bedrock coast, there is more bedrock, the coastline is comparatively smooth and straight, being affected by the faults and ocean dynamic process, which result in the larger fractal dimension.  相似文献   

富钴结壳分布的分形特征   总被引：8，自引：0，他引：8  

何高文 《海洋地质与第四纪地质》2001,21(1):89-92

利用我国在西太平洋国际海底两个海山获得的富钴结壳资料进行分形研究后发现，富钴结壳的分布具有变维分形分布的特点，结壳丰度呈二阶累计和分形分布，结壳厚度呈多阶不定累计和分形分布，通过计算得到了它们各自的分维数，进而对两个海山的结壳资源进行了初步评价。  相似文献   

Fractal Dimension and Fractals in Ocean Engineering   总被引：4，自引：0，他引：4  

Fu Yuhua Senior Engineer  China Offshore Oil Engineering Corporation  P. O. Box  Beijing 《中国海洋工程》1994,(3)

- This paper discusses the application of fractal dimension and fractals in ocean engineering. To handle some ocean environment problems, the existing fractal method, in which the fractal dimension is a constant, can be used. For some complicated problems in ocean engineering, this paper presents the concept of the variable dimension fractals (D = f(r)), i. e., the fractal dimension D is the function of characteristic scale r instead of a constant. By using variable dimension fractals, several deformation and stress states of offshore structures are described.  相似文献   

6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达     

陈启伟  刘广发  邱凤英 《台湾海峡》2004,23(3):314-317

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。  相似文献   

Characterization of zebrafish metallothionein gene promoter in a zebrafish caudal fin cell-line,SJD. 1     

Yan CH  Chan KM 《Marine environmental research》2002,54(3-5):335-339

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